Ascorbate Peroxidase (APx) Family
| Contact: | Marcia Margis-Pinheiro |
| Organization: | Universidade Federal do Rio Grande do Sul (UFRGS) |
| Website: | http://www.ufrgs.br/ufrgs/ |
| Source: | Teixeira, F.K., Menezes-Benaventa, L., Margis, R. and Margis-Pinheiro, M. (2004) Analysis of the Molecular Evolutionary History of the Ascobrate Peroxidase Gene Family: Inferences from the Rice Genome. J Mol Evol 59:761-770. Teixeira,F.K., Menezes-Benavente,L., Galvao,V.C., Margis,R., Margis-Pinheiro,M. (2006) Rice ascorbate peroxidase gene family encodes functionally diverse isoforms localized in different subcellular compartments. Planta DOI 10.1007/s00425-005-0214-8. |
| Criteria: | The annotation was performed using gene structure prediction programs (PROSITE, ProDom, FGENESH, and GeneMark program) and by comparison with the available ESTs and cDNAs sequences, which were obtained by searching the NCBI, TIGR, Rice GD databanks, and the library of 28,000 full-length rice cDNA (Kikuchi et al. (2003) Science 301:376-379). The predicted proteins were then analyzed for the presence of conserved APx domains and homology with typical APx sequences. |
| Gene Name: | OsAPx8 |
| Gene Description: | Thylakoid-bound Ascorbate Peroxidase encoding gene (5,8) |
| MSU Annotation: | OsAPx8 - Thylakoid-bound Ascorbate Peroxidase encoding gene 5,8, expressed |
| GenBank Genomic Acc: | AP004876 |
| GenBank Protein Acc: | BAC79363, Q69SV0 |
| Comment: | AB114856 |
| Structural Annotation: | |
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| Gene Name: | OsAPx1 |
| Gene Description: | Cytosolic Ascorbate Peroxidase encoding gene (1-8) |
| MSU Annotation: | OsAPx1 - Cytosolic Ascorbate Peroxidase encoding gene 1-8, expressed |
| GenBank Genomic Acc: | AC134232 |
| GenBank Protein Acc: | BAA08264, P93404 |
| Comment: | D45423 |
| Structural Annotation: | |
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| Gene Name: | OsAPx3 |
| Gene Description: | Peroxisomal Ascorbate Peroxidase encoding gene (5,8) |
| MSU Annotation: | OsAPx3 - Peroxisomal Ascorbate Peroxidase encoding gene 5,8, expressed |
| GenBank Genomic Acc: | AL606634 |
| GenBank Protein Acc: | AAQ88105, Q6TY83 |
| Comment: | AY382617 |
| Structural Annotation: | |
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| Gene Name: | OsAPx7 |
| Gene Description: | Stromal Ascorbate Peroxidase encoding gene (5,8) |
| MSU Annotation: | OsAPx7 - Stromal Ascorbate Peroxidase encoding gene 5,8, expressed |
| GenBank Genomic Acc: | AL606706 |
| GenBank Protein Acc: | BAC79362, Q7XJ02 |
| Comment: | AB114855, AK103344 |
| Structural Annotation: | |
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| Gene Name: | OsAPx2 |
| Gene Description: | Cytosolic Ascorbate Peroxidase encoding gene (4,5,6,8) |
| MSU Annotation: | OsAPx2 - Cytosolic Ascorbate Peroxidase encoding gene 4,5,6,8, expressed |
| GenBank Genomic Acc: | AP005199 |
| GenBank Protein Acc: | BAA20889, Q9FE01 |
| Comment: | AB053297 |
| Structural Annotation: | |
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| Gene Name: | OsAPx4 |
| Gene Description: | Peroxisomal Ascorbate Peroxidase encoding gene (5,8,9) |
| MSU Annotation: | OsAPx4 - Peroxisomal Ascorbate Peroxidase encoding gene 5,8,9, expressed |
| GenBank Genomic Acc: | AP003912 |
| GenBank Protein Acc: | Q6ZJJ1 |
| Comment: | AK070842 |
| Structural Annotation: | |
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| Gene Name: | OsAPx6 |
| Gene Description: | Stromal Ascorbate Peroxidase encoding gene (5,8) |
| MSU Annotation: | OsAPx6 - Stromal Ascorbate Peroxidase encoding gene 5,8, expressed |
| GenBank Genomic Acc: | DP000011 |
| GenBank Protein Acc: | ABA96617, P0C0L1 |
| Comment: | AK061107,AK099201 |
| Structural Annotation: | |
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| Gene Name: | OsAPx5 |
| Gene Description: | Stromal Ascorbate Peroxidase encoding gene (5,8) |
| MSU Annotation: | OsAPx5 - Stromal Ascorbate Peroxidase encoding gene 5,8 |
| GenBank Genomic Acc: | DP000011 |
| GenBank Protein Acc: | ABA96618, P0C0L0 |
| Comment: | AK073910 |
| Structural Annotation: | |
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References: 1 Morita,S., Kaminaka,H., Yokoi,H., Masumura,T. and Tanaka,K. (1997) Cloning and characterization of cytosolic ascorbate peroxidase cDNA from rice (PGR97-012). Plant Physiol. 113:306. 2 Agrawal,G.K., Rakwal,R., Yonekura,M., Kubo,A. and Saji,H. (2002) Proteome analysis of differentially displayed proteins as a tool for investigating ozone stress in rice (Oryza sativa L.) seedlings. Proteomics 2:947-959. 3 Sato,Y., Murakami,T., Funatsuki,H., Matsuba,S., Saruyama,H. and Tanida,M. (2001) Heat shock-mediated APX gene expression and protection against chilling injury in rice seedlings. J. Exp. Bot. 52:145-151. 4 Agrawal,G.K., Jwa,N.S., Iwahashi,H. and Rakwal,R. (2003) Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling. Gene 322:93-103. 5 Teixeira,F.K., Menezes-Benavente,L., Margis,R. and Margis-Pinheiro,M. (2004) Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family: inferences from the rice genome. J. Mol. Evol. 59:761-770. 6 Lu,Z., Takano,T. and Liu,S. (2005) Purification and characterization of two ascorbate peroxidases of rice (Oryza sativa L.) expressed in Escherichia coli. Biotechnol. Lett. 27:63-67. 7 Tsai,Y.C., Hong,C.Y., Liu,L.F. and Kao,C.H. (2005) Expression of ascorbate peroxidase and glutathione reductase in roots of rice seedlings in response to NaCl and H2O2. J. Plant Physiol. 162:291-299. 8 Teixeira,F.K., Menezes-Benavente,L., Galvao,V.C., Margis,R., Margis-Pinheiro,M. (2006) Rice ascorbate peroxidase gene family encodes functionally diverse isoforms localized in different subcellular compartments. Planta DOI 10.1007/s00425-005-0214-8. 9 Rice Full-Length cDNA Consortium (2003) Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice. Science 301 (5631), 376-379. |
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This work is supported by grants (DBI-0321538/DBI-0834043) from the National Science Foundation and funds from the Georgia Research Alliance, Georgia Seed Development, and University of Georgia.