Rice HKT transporters

   
Contact: Olivier Cotsaftis
Organization: Australian Centre for Plant Functional Genomics (ACPFG) and University of Adelaide
Source: Platten JD, Cotsaftis O, Berthomieu P, Bohnert H, Davenport RJ, Fairbairn, DJ, Horie T, Leigh RA, Lin HX, Luan S, Maser P, Pantoja O, Rodriguez-Navarro A, Schachtman DP, Schroeder JI, Sentenac H, Uozumi N, Very AA, Zhu JK, Dennis ES, Tester M. (2006) Nomenclature for HKT transporters, key determinants of plant salinity tolerance. Trends Plant Sci. 11(8):372-4
Criteria: Phylogenetic analysis of the HKT family of Na+ and K+/Na+ in higher plants. Note that one member of the family, OsHKT2;2, which was discovered in the Pokkali cultivar (indica) is absent from the Nipponbare genome and is not displayed here.
   

Chr4:30548314..30545885

Gene Name: OsHKT1;2
Alternate Gene Name: HKT5
Gene Description: Na+ transporter
GenBank Genomic Acc: AJ506745
Comment: Q-PCR forward primer: 5'-ACATCACAGCATCTGCCATT-3'
Q-PCR reverse primer: 5'-AGAAACAGCGTCTGAAGAAACA-3'
Structural Annotation:  

   

LOC_Os01g20160

Gene Name: OsHKT1;5
Alternate Gene Name: HKT8; SKC1
Gene Description: Na+ transporter
MSU Annotation: OsHKT1;5 - Na+ transporter, expressed
GenBank Genomic Acc: AP003567
Mutant Line/Info: Postech D09425
Comment: AK108663
BAB93392
Q-PCR forward primer: 5'-ACGACCCCATCAACTACAGCGTCC-3'
Q-PCR reverse primer: 5'-ACGACCCCATCAACTACAGCGTCC-3'
Structural Annotation:  

   

LOC_Os01g34850

Gene Name: OsHKT2;3
Alternate Gene Name: HKT3
Gene Description: Na+ transporter
MSU Annotation: OsHKT2;3 - Na+ transporter, expressed
GenBank Genomic Acc: AJ491819
Mutant Line/Info: Tos17 ND3058 (FST: AG213420)
Comment: AJ491820
CAD37187
Q-PCR forward primer: 5'-GSCTTCAACACCACAGTGATAATG-3'
Q-PCR reverse primer: 5'-GTAGCCAGTGGACAACCCAATG-3'
(same as for OsHKT2;4)
Structural Annotation:  

   

LOC_Os02g07830

Gene Name: OsHKT1;3
Alternate Gene Name: HKT6
Gene Description: Na+ transporter
MSU Annotation: OsHKT1;3 - Na+ transporter, expressed
GenBank Genomic Acc: AJ491817
Comment: AJ491818
CAD37185
Q-PCR forward primer: 5'-GCTTACTTTGCCCTGATCTCCT-3'
Q-PCR reverse primer: 5'-TGAATACCTCACCACCAATC-3'
Structural Annotation:  

   

LOC_Os04g51820

Gene Name: OsHKT1;1
Alternate Gene Name: HKT4
Gene Description: Na+ transporter
MSU Annotation: OsHKT1;1 - Na+ transporter, expressed
GenBank Genomic Acc: AJ491815
Mutant Line/Info: Tos17 ND8018 (FST: AG207416)
Comment: AJ491816
CAD37183
Q-PCR primer forward: 5'-GAGCACTGTGGAGGAATTTTACCG -3'
Q-PCR primer reverse: 5'-TAGTGAGTAGCCTACATTGCCGAAA-3'
Structural Annotation:  

   

LOC_Os04g51830

Gene Name: OsHKT1;4
Alternate Gene Name: HKT7
Gene Description: Na+ transporter
MSU Annotation: OsHKT1;4 - Na+ transporter, expressed
GenBank Genomic Acc: AJ491853
Comment: AJ491818
CAD37197
Q-PCR forward primer: 5'-GCGACTCTGGCAAACTGATA-3'
Q-PCR reverse primer: 5'-GGTTCCTGTCTATGTGAAAATGAATA-3'
Structural Annotation:  

   

LOC_Os06g48800

Gene Name: OsHKT2;4
Alternate Gene Name: HKT9
Gene Description: Na+ transporter
MSU Annotation: OsHKT2;4 - Na+ transporter
GenBank Genomic Acc: AJ491854
Mutant Line/Info: Tos17 NE7041 (FST: AG209602)
Comment: AJ491855
CAD37199
Q-PCR forward primer: 5'-GSCTTCAACACCACAGTGATAATG-3'
Q-PCR reverse primer: 5'-GTAGCCAGTGGACAACCCAATG-3'
(same as for OsHKT2;3)
Structural Annotation:  

   

LOC_Os06g48810

Gene Name: OsHKT2;1
Alternate Gene Name: HKT1
Gene Description: Na+ transporter
MSU Annotation: OsHKT2;1 - Na+ transporter, expressed
GenBank Genomic Acc: AJ491852
Mutant Line/Info: Tos17 NC2534 (FST: AG205615)
Comment: AB061311
BAB61789
Q-PCR forward primer: 5'-GTTAATTTTGTTGTTCTAGC-3'
Q-PCR reverse primer: 5'-ATGAGGCTGGAAAGTGTCAG-3'
Structural Annotation:  

 

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This work is supported by grants (DBI-0321538/DBI-0834043) from the National Science Foundation and funds from the Georgia Research Alliance, Georgia Seed Development, and University of Georgia.