Adenylyl Sulfate Reductase-like

   
Contact: Norma Houston
Organization: North Carolina State University
Website: http://www.ncsu.edu/
Source: Norma L. Houston, Chuanzhu Fan, Qiu-Yun Xiang, Jan-Michael Schulze, Rudolf Jung, and Rebecca S. Boston (2005) Phylogenetic Analyses Identify 10 Classes of the Protein Disulfide Isomerase Family in Plants, Including Single-Domain Protein Disulfide Isomerase-Related Proteins. Plant Physiology 137: 762-778
Criteria: Thioredoxin domains from PDIL amino acid alignments formed a distinct, well-supported clade within the thioredoxin gene family. The adenosine 5'- phosphosulfate reductase-like (APRL) amino acid sequences formed a distinct subclade that was strongly supported within the PDIL clade. Three members of the APRL subclade are close homologs of bifunctional proteins with reductase activity typical of thioredoxins as well as adenylyl sulfate reductase (APR) activity. The two enzyme activities are encoded in adjacent domains (Gutierrez-Marcos et al., 1996; Setya et al., 1996; Wray et al., 1998; Prior et al., 1999).
   

LOC_Os02g51850
Gene Name: OsAPRL3
Gene Description: Oryza sativa adenosine 5'-phosphosulfate reductase-like (OsAPRL3)
MSU Annotation: OsAPRL3 adenosine 5'-phosphosulfate reductase-like OsAPRL3, expressed
GenBank cDNA Acc: XM_467860
GenBank Protein Acc: XP_467860
Comment: Predicted Localization1: S
Domain Composition2: thioredoxin (1)
Structural Annotation:  

   

LOC_Os03g59170
Gene Name: OsAPRL5
Gene Description: Oryza sativa adenosine 5'-phosphosulfate reductase-like (OsAPRL5)
MSU Annotation: OsAPRL5 adenosine 5'-phosphosulfate reductase-like OsAPRL5, expressed
GenBank Protein Acc: XP_470023
Comment: AK073308
Predicted Localization1: S
Domain Composition2: thioredoxin (1)
Structural Annotation:  

   

LOC_Os06g11740
Gene Name: OsAPRL2
Gene Description: Oryza sativa adenosine 5'-phosphosulfate reductase-like (OsAPRL2)
MSU Annotation: OsAPRL2 adenosine 5'-phosphosulfate reductase-like OsAPRL2, expressed
GenBank cDNA Acc: AY739306
GenBank Protein Acc: AAX14677
Comment: Predicted Localization1: O
Domain Composition2: thioredoxin (1)
Structural Annotation:  

   

LOC_Os07g32570
Gene Name: OsAPRL1
Gene Description: Oryza sativa adenosine 5'-phosphosulfate reductase-like (OsAPRL1)
MSU Annotation: OsAPRL1 adenosine 5'-phosphosulfate reductase-like OsAPRL1, expressed
GenBank cDNA Acc: XM_478340
GenBank Protein Acc: XP_478340
Comment: Predicted Localization1: O
Domain Composition2: thioredoxin (1), PAPS_reductase (1)
Structural Annotation:  

   

LOC_Os08g31814
Gene Name: OsAPRL4
Gene Description: Oryza sativa adenosine 5'-phosphosulfate reductase-like (OsAPRL4)
MSU Annotation: OsAPRL4 adenosine 5'-phosphosulfate reductase-like OsAPRL4, expressed
GenBank cDNA Acc: AY739307
GenBank Protein Acc: AAX14678
Comment: Predicted Localization1: S
Domain Composition2: thioredoxin (1)
Structural Annotation:  

   

LOC_Os12g35640
Gene Name: OsAPRL6
Gene Description: Oryza sativa adenosine 5'-phosphosulfate reductase-like (OsAPRL6)
MSU Annotation: OsAPRL6 adenosine 5'-phosphosulfate reductase-like OsAPRL6, expressed
Comment: Predicted Localization1: S'
Domain Composition2: thioredoxin (1)
This is a predicted gene annotated by Gramene (GRMT00000182570)
Structural Annotation:  

 

1 S, Secretory pathway assignment by TargetP; (Emanuelsson et al., 2000) reliability value 0.6; S', secretory pathway assignment reliability value <0.6; O, other localization predicted.

2 Domains predicted by CDD (Conserved Domain Database) followed bynumber of each domain

References:
Gutierrez-Marcos JF, Roberts MA, Campbell EI, Wray JL (1996) Three members of a novel small gene-family from Arabidopsis thaliana able to complement functionally an Escherichia coli mutant defective in PAPS reductase activity encode proteins with a thioredoxin-like domain and "APS reductase" activity. Proc Natl Acad Sci USA 93: 13377-13382

Prior A, Uhrig JF, Heins L, Wiesmann A, Lillig CH, Stoltze C, Soll J, Schwenn JD (1999) Structural and kinetic properties of adenylyl sulfate reductase from Catharanthus roseus cell cultures. Biochim Biophys Acta 1430: 25-38

Setya A, Murillo M, Leustek T (1996) Sulfate reduction in higher plants: molecular evidence for a novel 5'-adenylylsulfate reductase. Proc Natl Acad Sci USA 93: 13383-13388

Wray JL, Campbell EI, Roberts MA, Gutierrez-Marcos JF (1998) Redefining reductive sulfate assimilation in higher plants: a role for APS reductase, a new member of the thioredoxin superfamily? Chem Biol Interact 109: 153-167

NSF Logo This work is supported by grants (DBI-0321538/DBI-0834043) from the National Science Foundation.